MBIS Video: Bulk RNA-Seq Data Analysis using CLC Genomics Workbench

The following video outlines the topics that will be covered in this course.

Note: HSLS MBIS videos are only available to users with a valid Pitt username and password.

If you're having trouble playing this embedded video, you can also watch "Intro to Topics -- Bulk RNA-Seq Data Analysis using CLC Genomics Workbench" directly on Panopto.

In the following video, a brief overview of available software for RNA-Seq data analysis is presented.

Note: HSLS MBIS videos are only available to users with a valid Pitt username and password.

If you're having trouble playing this embedded video, you can also watch "Software Overview -- Bulk RNA-Seq Data Analysis using CLC Genomics Workbench" directly on Panopto.

The RNA-Seq dataset taken from a published Cell paper is described in the following video.

Note: HSLS MBIS videos are only available to users with a valid Pitt username and password.

If you're having trouble playing this embedded video, you can also watch "RNA-Seq Dataset -- Bulk RNA-Seq Data Analysis using CLC Genomics Workbench" directly on Panopto.

Data Download

• ENA Browser for SRP113442 - link to download FASTQ reads
• Metadata file (.xlsx) - link to download a metadata file with sample information, and was used to associate FASTQ reads

Reference

• Pulikkan JA, Hegde M, Ahmad HM, et al. CBFβ-SMMHC Inhibition Triggers Apoptosis by Disrupting MYC Chromatin Dynamics in Acute Myeloid Leukemia. Cell. 2018;174(1):172-186.e21. doi:10.1016/j.cell.2018.05.048

GEO Record

• GSE101788 : CBFb-SMMHC inhibition triggers apoptosis by disrupting MYC chromatin dynamics in acute myeloid leukemia [RNA-seq]