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HSLS MolBio Workshops: Bulk RNA-Seq: CLC Genomics

Information about hands-on bioinformatics classes

Workshop Objective

This is a 4-hour workshop. The morning session (10am - 12pm) provides a brief introduction to techniques, platforms, and methods used in bulk RNA Seq experiments.  The afternoon session (1pm - 3pm) focuses on hands-on data analysis practice using HSLS-licensed CLC Genomics Workbench.

Participants will learn how to

  • import NGS reads from an NCBI GEO dataset
  • align the reads to a reference genome
  • identify differentially expressed genes from RNA Seq data
  • visualize and summarize the output of RNA Seq result

Target Audience:

Experimental biologists seeking to analyze bulk RNASeq data generated through experiments or retrieved from a publication or a repository such as GEO. The software covered in the workshop operates through a user-friendly, point-and-click graphical user Interface, so neither computer programming experience nor familiarity with command line interface is required.

Publications from Pitt Showing Software Applications

Attribution

Please include the following statement in the acknowledgments section for all publications, posters, and presentations: 

CLC Genomics Workbench software licensed through the Molecular Biology Information Service of the Health Sciences Library System, University of Pittsburgh was used for data analysis.

The Health Sciences Library System supports the Health Sciences at the University of Pittsburgh.

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